Bioluminescence imaging of these plants after agro-infiltration with TuYV-N65-NL allowed the detection of the virus in systemically infected leaves. benthamiana plants expressing the C-terminal domain of split NanoLuc. For the detection of split NanoLuc, we constructed transgenic N. Bioluminescence imaging detected TuYV-NL in inoculated and systemically infected leaves. RT-PCR analysis indicated that the recombinant sequences were stable in N. perfoliata but reduced systemic infection and lower accumulation in A. ELISA confirmed systemic infection and similar accumulation of the recombinant viruses in N. Wild-type and recombinant viruses were agro-infiltrated in Nicotiana benthamiana, Montia perfoliata, and Arabidopsis thaliana. For this, TuYV was genetically tagged by replacing the C-terminal part of the RT protein with full-length NanoLuc (TuYV-NL) or with the N-terminal domain of split NanoLuc (TuYV-N65-NL). We used the NanoLuc luciferase bioluminescent reporter system to detect turnip yellows virus (TuYV) in infected plants.
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